Subjects: Biology >> Zoology submitted time 2017-11-07 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究自然霉变饲粮中镰刀菌毒素对断奶仔猪脾脏抗氧化能力和白细胞介素−1β（IL-1β）、白细胞介素−6（IL-6）分布和表达的影响。选择35日龄平均体重为（8.45±0.94） kg的健康三元杂交（杜×长×大）断奶仔猪母猪40头，随机分为2组，每组20头。对照组饲喂基础饲粮，镰刀菌毒素组饲喂含镰刀菌毒素[玉米赤霉烯酮（ZEN）0.90 mg/kg；呕吐毒素（DON）1.43 mg/kg，烟曲霉毒素（FUM）5.85 mg/kg]的试验饲粮。预试期7 d，正试期35 d。结果表明：1）与对照组相比，镰刀菌毒素显著降低了断奶仔猪血清和脾脏谷胱甘肽过氧化物酶（GSH-Px）和总超氧化物歧化酶（T-SOD）活性（P<0.05），显著升高了丙二醛（MDA）含量（P<0.05）。2）镰刀菌毒素使断奶仔猪脾脏白髓区明显变小，红髓区扩张且出现近圆形小空洞，动脉周围淋巴鞘中淋巴细胞数量较少。3）镰刀菌毒素导致断奶仔猪脾脏IL-1β和IL-6阳性细胞主要集中于白髓边缘，且靠近血窦的地方阳性点更多。4）与对照组相比，镰刀菌毒素显著升高了断奶仔猪脾脏IL-1β和IL-6 mRNA相对表达量（P<0.05）。由此可见，饲粮中镰刀菌毒素显著影响断奶仔猪血清和脾脏抗氧化能力，并通过改变脾脏IL-1β和IL-6的分布和表达，降低脾脏的免疫功能。
Subjects: Biology >> Zoology submitted time 2017-10-11 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究自然霉变饲粮中镰刀菌毒素对断奶仔猪脾脏抗氧化能力和白细胞介素−1β（IL-1β）、白细胞介素−6（IL-6）分布和表达的影响。选择35日龄平均体重为（8.45±0.94） kg的健康三元杂交（杜×长×大）断奶仔猪母猪40头，随机分为2组，每组20头。对照组饲喂基础饲粮，镰刀菌毒素组饲喂含镰刀菌毒素[玉米赤霉烯酮（ZEN）0.90 mg/kg；呕吐毒素（DON）1.43 mg/kg，烟曲霉毒素（FUM）5.85 mg/kg]的试验饲粮。预试期7 d，正试期35 d。结果表明：1）与对照组相比，镰刀菌毒素显著降低了断奶仔猪血清和脾脏谷胱甘肽过氧化物酶（GSH-Px）和总超氧化物歧化酶（T-SOD）活性（P<0.05），显著升高了丙二醛（MDA）含量（P<0.05）。2）镰刀菌毒素使断奶仔猪脾脏白髓区明显变小，红髓区扩张且出现近圆形小空洞，动脉周围淋巴鞘中淋巴细胞数量较少。3）镰刀菌毒素导致断奶仔猪脾脏IL-1β和IL-6阳性细胞主要集中于白髓边缘，且靠近血窦的地方阳性点更多。4）与对照组相比，镰刀菌毒素显著升高了断奶仔猪脾脏IL-1β和IL-6 mRNA相对表达量（P<0.05）。由此可见，饲粮中镰刀菌毒素显著影响断奶仔猪血清和脾脏抗氧化能力，并通过改变脾脏IL-1β和IL-6的分布和表达，降低脾脏的免疫功能。
Subjects: Biology >> Zoology submitted time 2017-10-10 Cooperative journals: 《动物营养学报》
Abstract： The aim of this study was to investigate the effects of different levels of zearalenone (ZEA) on serum enzymes and metabolite and intestinal morphology of weaned gilts. Forty healthy weaned gilts (Duroc譒andrace譟orkshire) aged 28 days with body weight of (14.01�.86) kg were randomly allocated into 4 groups (10 replicates per group, 1 pig per replicate). Weaned gilts in the control group were fed a basal diet, and those in the experiment groups 1, 2 and 3 were fed ZEA contaminated diet with 0.5, 1.0 and 1.5 mg/kg ZEA supplementation, respectively. The experiment included 10 days pretrial period and then 35 days test period. The results showed as follows: 1) compared with the control group, the activities of aspartate aminotransferase, alkaline phosphatase and lactate dehydrogenase in experimental groups were significantly increased (P<0.05), serum total cholesterol content in experimental group 2 was significantly increased (P<0.05), and the contents of serum urea nitrogen and high-density lipoprotein in experimental group 3 were significantly increased (P<0.05), while the serum total protein content was significantly decreased (P<0.05). 2) Compared with the control group, the villus height and crypt depth of duodenum and jejunum in experimental groups were significantly increased (P<0.05). In the experimental groups 1 and 2, the ratio of villus height to crypt depth in duodenum was significantly increased (P<0.05), the ratio of villus height to crypt depth in jejunum was significantly decreased (P<0.05), and the villus height and the ratio of villus height to crypt depth in jejunum were significantly increased (P<0.05). 3) Compared with the control group, the villus height in duodenum of experimental groups was significantly increased, and the arrangement of them were loose and disordered. The number of small intestinal glands was decreased and the mucosal thickness became thinner. 4) Compared with the control group, the villus height and the number of small intestinal glands in jejunum of experimental groups was decreased, and the jejunal epithelium of experimental groups 2 and 3 was denuded and the lamina propria was exposed. 5) Compared with the control group, the villus height in ileum of experimetal groups 2 and 3 was increased, and the number of diffuse lymphatic cells in the submucosa was increased. Therefore, dietary ZEA (0.5 to 1.5 mg/kg) can change the liver metabolism and intestinal morphology of weaned gilts, thereby affecting the healthy growth of weaned gilts.
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